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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • wheat
GE trait
  • quality
Country
  • UK, Spain, Australia

Results

Equivalence
  • no effect

Comparative field performance over 3 years and two sites of transgenic wheat lines expressing HMW subunit transgenes

Shewry PR, Powers S, Field JM, Fido RJ, Jones HD, Arnold GM, West J, Lazzeri PA, Barcelo P, Barro F, Tatham AS, Bekes F, Butow B, Darlington H.
Theoretical and Applied Genetics. 2006 June. 113(1):128-136

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PMID: 16783593 DOI: 10.1007/s00122-006-0279-1 ISSN: 0040-5752

Abstract

A series of transgenic wheat lines expressing additional high molecular weight (HMW) subunit genes and the corresponding control lines were grown in replicate field trials at two UK sites (Rothamsted Research, approximately 50 km north of London and Long Ashton, near Bristol) over 3 years (1998, 1999, 2000), with successive generations of the transgenic lines (T3, T4, T5) being planted. Four plots from each site were used to determine grain dry weight, grain nitrogen, dough strength (measured as peak resistance by Mixograph analysis) and the expression levels of the endogenous and "added" subunits. Detailed statistical analyses showed that the transgenic and non-transgenic lines did not differ in terms of stability of HMW subunit gene expression or in stability of grain nitrogen, dry weight or dough strength, either between the 3 years or between sites and plots. These results indicate that the transgenic and control lines can be regarded as substantially equivalent in terms of stability of gene expression between generations and environments.

Keywords

wheat, stability, transgene, Rothamsted

Funding

Funding source
  • Biological Sciences Research Council, UK
  • Zeneca (Syngenta)
Funding country
  • UK
Funding type
  • government
  • industry: same

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Cite this study

MLA

Shewry PR, Powers S, Field JM, Fido RJ, Jones HD, Arnold GM, West J, Lazzeri PA, Barcelo P, Barro F, Tatham AS, Bekes F, Butow B, Darlington H.. "Comparative field performance over 3 years and two sites of transgenic wheat lines expressing HMW subunit transgenes." Theoretical and Applied Genetics 113.1 (2006): 128-136. Web. 23 Nov. 2024.

APA

Shewry PR, Powers S, Field JM, Fido RJ, Jones HD, Arnold GM, West J, Lazzeri PA, Barcelo P, Barro F, Tatham AS, Bekes F, Butow B, Darlington H.. (2006). Comparative field performance over 3 years and two sites of transgenic wheat lines expressing HMW subunit transgenes. Theoretical and Applied Genetics, 113(1), 128-136. doi:10.1007/s00122-006-0279-1

Please verify citations before use, citations are automatically generated based on information stored within the GENERA database and therefore may or may not be correct.